Abstract 3962: Hematopoietic Endothelial Progenitor Cells Enhance Motor Function And Motor Map Integrity Following Cerebral Ischemia.
Stroke is the leading cause of disability in the Unites States resulting in upper extremity motor impairments. The present study examined the efficacy of systemically administered hematopoietic endothelial progenitor cells (EPCs) for ameliorating motor impairments and cortical dysfunction following cerebral ischemia.
Methods: Baseline motor performance of Forty one male adult rats was established on a single pellet reaching task. Animals were assigned to one of five experimental conditions that counterbalanced baseline motor performance. Animals in the Stroke condition (n=7) received infusion of endothelin-1 onto the middle cerebral artery contralateral to the preferred paw. Animals in the PRE (n=7) and POST (n=7) conditions received the same infusion of endothelin-1 one but also received ten million EPCs injected into the tail vein either thirty minutes before (PRE) or immediately after (POST) infusion. The EPC’s were enriched from the bone marrow of a separate group of donor rats using nanoparticles tagged with LIN negative and CD90 markers. SHAM (n=9) animals only received EPC infusion and CONTROLS received no stroke or EPC infusion. One, three and five weeks following stroke all animals were tested on the skilled reaching task after which intracortical microstimulation was used to derive maps of forelimb movement representations within the motor cortex contralateral to the preferred paw. All assessments were performed in a blinded manner.
Results: showed POST animals performed significantly better on single pellet reaching than animals in all other stroke conditions on weeks 3 and 5. Control animals scored a reaching accuracy of 41±8.1% and 46±11% at 3 and 5 weeks, respectively. Stroke decreased this task to 12±2.1% and 18±2.8%, respectively (p<0.05, compared to controls at both time points). Injection of EPC’s following reperfusion led to an increase in reaching accuracy to 27±2.6% and 32±2.4%, at 3 and 5 weeks, respectively (p<0.05, compared to stroked non-EPC cohort at 5 weeks). Further, POST animals had significantly larger forelimb motor maps than animals in all other stroke conditions. Infarct size did not significantly differ between the stroke conditions. The results show that post stroke treatment of EPCs does not reduce infarct size but does enhance the functional integrity of residual cortical tissue that in turn supports enhanced behavioral outcome.
- © 2012 by American Heart Association, Inc.