Abstract TP215: Leukocyte RNA Expression Is Altered Prior To Hemorrhagic Transformation In Ischemic Stroke
Background: Hemorrhagic transformation (HT) is a major complication of ischemic stroke that worsens outcomes and increases mortality. In ischemic stroke circulating leukocytes may contribute to blood brain barrier disruption, which is an important component of HT. In this study we sought to determine whether leukocytes express RNA differently in ischemic strokes that latter develop HT, and thus identify molecules and pathways important for the pathogenesis of HT in human stroke.
Methods: Blood samples were collected within 3 hours of ischemic stroke onset prior to thrombolytic therapy. No subjects had HT prior to sample collection. RNA from circulating leukocytes was isolated and processed on Affymetrix U133 Plus2.0 microarrays. Ischemic strokes that developed HT (n=11) were compared to matched ischemic strokes without HT (n=33) and controls (n=14). Genes with a Benjamini Hochberg corrected p-value <0.05 and fold change >
- © 2012 by American Heart Association, Inc.