Abstract WMP39: Co-transplantation of Neural Stem cells and Vascular Progenitor Cells Enhances Post-ischemic Recovery in Cerebral Ischemia in Rats
Introduction: Neuronal and vascular regeneration is important for post-ischemic recovery. In this study we investigated whether co-transplantation of neural stem cells (NSCs) and vascular progenitor cells (VPCs) could promote the therapeutic efficacy of stem cell based therapy after transient middle cerebral artery occlusion (tMCAO).
Methods: tMCAO was generated in adult male SD rats. One day after MCAO, NPCs (1x106), VPCs (1x106) or NPCs (5 x105) and VPCs (5x105) were transplanted into the cortical perifocal region. Neurobehavioral outcomes, infarct volume, cell differentiation, angiogenesis and neurogenesis were examined within 14 days following tMCAO.
Results: MRI analysis and behavioral tests showed that co-transplantation of NSCs and VPCs significantly decreased infarct volume and improved neurobehavioral recovery at 7 and 14 days after ischemia compared to NPCs group and VPCs group (p<0.05). Immunostaining showed that angiogenesis and neurogenesis were increased in ipsilateral hemisphere in NPCs/VPCs co-transplantation rats compared to single cell transplanted groups (p<0.05). Fourteen days after ischemia, NPCs and VPCs were detected in the ipsilateral cortex, which differentiated into neurons, astrocytes and incorporated into host blood vessels as endothelial cells (ECs) and smooth muscle cells (SMCs).
Conclusion: We demonstrated that co-transplantation of NPCs and VPCs had great benefits for post-ischemic recovery. Our findings suggest that combined transplantation of different types of stem cells may constitute a novel therapeutic strategy for improving the treatment of ischemic diseases.
- © 2012 by American Heart Association, Inc.