Abstract 157: MicroRNA Expression Signature in Human Brain Arteriovenous Malformations and Brain Hemangioblastoma
Objectives: Small noncoding microRNAs (miRNAs) play critical roles in many biological and pathological processes and are differentially expressed in normal tissue and diseased tissue. miRNAs expression and function in brain vascular diseases are unknown. In this study, we aim to investigate the miRNAs signature in human brain arteriovenous malformations (BAVM) and hemangioblastoma.
Methods: Total miRNA from the surgical specimen of BAVM patients (n=6), hemangioblastoma patients (n=5), and healthy individuals (n =5) were assessed using qPCR and Exiqon Human Panel. 739 miRNAs were validated with qRT-PCR analysis to establish the miRNA expression signature. To predict potential miRNA targets and analyze their functions, the preliminary qRT-PCR data was analyzed by GenEx and Ingenuity Pathway Analysis software.
Results: miRNAs were differentially expressed in BAVM and hemangioblastoma patients vs. healthy controls. The miRNA expression levels of 85 and 166 miRNAs were significantly different (p<0.05) in BAVM and in brain hemangioblastoma patients compared to healthy controls, respectively. 93 miRNAs expressed significantly differently (p<0.05 and passed Bonferroni correction) in the BAVM patients compared to hemangioblastoma patients; Principal component analysis demonstrated a distinction between BAVM and hemangioblastoma vs. healthy controls based on the miRNAs. Bioinformatic analysis indicated that the most of miRNAs were involved in notch signal pathway and angiogenesis.
Conclusions: This study showed that there was a distinct miRNA expression signature in brain vascular diseases. Our findings provided a valuable clue on the identification of miRNAs’ function in cerebrovascular pathologic processes and the development of therapeutic targets.
Author Disclosures: J. Huang: None. W. Zhu: None. J. Song: None. Q. An: None. Y. Wang: None. S. Zhang: None. T. Xu: None. G. Tang: None. Y. Liu: None. Y. Li: None. Y. Wang: None. G. Yang: None.
- © 2014 by American Heart Association, Inc.