Abstract 190: Allogeneic Marrow Stromal Cells Show Differences in Activation After Exposure to Blood From Healthy Subjects versus Stroke Patients
Background: Systemic administration of marrow stromal cells (MSCs) leads to the release of a broad panel of cytokines and growth factors that may stimulate repair after stroke. However, the activation of MSCs may differ depending on the external environment they encounter after systemic injection. We explored the question whether the secretomes of MSCs, once exposed to systemic blood, differs depending on whether blood is from stroke patients versus healthy controls.
Methods: Clinical grade bone marrow MSCs from a healthy donor at passage 2 were thawed and re-suspended in PBS. Peripheral blood from healthy subjects (n=4) and stroke patients (n=3) was collected. Blood from stroke patients was collected at 24 hrs after symptom onset. Whole blood or plasma was added to wells. PBS was then added with or without 200,000 MSCs. At various time points, aliquots were collected and probed for cytokines using a MAGPIX reader.
Results: Placing MSCs in blood from healthy or stroke patients led to a differential release of cytokines (Fig 1). There were no changes in cytokines levels over time from wells that contained MSCs plus blood derived from healthy controls. However, there were significant increases in IL-8, IL-10 and VEGF levels in wells that contained MSCs plus blood from stroke patients (Fig 1). The increase in TNF-α in wells that contained blood alone from stroke patients was reduced by the addition of MSCs. In wells that contained MSCs plus plasma derived from stroke patients, there were very similar cytokine changes compared with wells that contained MSCs plus plasma derived from healthy controls (data not shown).
Conclusion: The components of circulatory blood in stroke patients may be important to activate MSCs to release soluble factors that could modify brain recovery after stroke. Upon systemic administration, the exposure of MSCs to the microenvironment of blood might lead to different activation patterns depending on the health status of the individual.
Author Disclosures: K. Parsha: None. W. Guerrero: None. B. Yang: None. O. Mir: None. P. Hanley: None. A. Gee: None. S.I. Savitz: None.
- © 2014 by American Heart Association, Inc.