Abstract 196: Human Recombinant Peptide (RCP) Sponge Enables Novel Noninvasive Cell Therapy for Ischemic Stroke
Background: The bone marrow stromal cells (BMSCs) have the potential to improve neurologic function when transplanted into animal models of ischemic stroke. However, it is unclear through which route the BMSC should be delivered to yield the maximal therapeutic benefits safely. Using genetic engineering technique, the authors have developed the recombinant peptide (RCP) sponge of human collagen. Therefore, this study was aimed to assess whether the RCP sponge of human collagen could act as a noninvasive, valuable scaffold in cell therapy for ischemic stroke.
Methods: The BMSCs were harvested from GFP-transgenic rats and were cultured in the RCP sponge. The rats were subjected to permanent middle cerebral artery (MCA) occlusion. Vehicle, BMSC suspension, or the BMSC-RCP sponge construct was transplanted onto the ipsilateral intact neocortex at 7 days after the insult. Motor function was serially evaluated throughout the experiments. The distribution and fate of engrafted cells were examined at 6 weeks after BMSC transplantation.
Results: The BMSCs proliferated in the RCP sponge in vitro. Transplantation of BMSC suspension or the BMSC-RCP sponge construct equally promoted functional recovery, when compared with the vehicle group. RCP sponge provoked no inflammatory reactions in the host brain. Histological analysis revealed that the BMSC migrated and differentiated into the neural cells in the infarct brain when transplanted using the RCP sponge.
Conclusion: These findings suggest that surgical transplantation of tissue-engineered BMSC onto the infarct neocortex may enhance functional recovery. RCP sponge can be a promising candidate for the animal protein-free scaffolds in cell therapy for ischemic stroke.
Author Disclosures: M. Miyamoto: None. S. Kuroda: None. K. Nakamura: None. T. Yamauchi: None. M. Ito: None. H. Saito: None. H. Shichinohe: None. T. Sasaki: None. K. Houkin: None.
- © 2014 by American Heart Association, Inc.