Abstract 204: Xe-Loaded Liposome Reduces Bleeding With Neuroprotection From Early Injury in Subarachnoid Hemorrhagic Stroke
Background: Cell death, especially apoptosis occurs in subarachnoid hemorrhage (SAH) due to rapid and massive brain injury from the initial bleeding. Xe-loaded echogenic liposomes (Xe-ELIP) have been reported to have hemostatic effects in SAH. The goals of this study were to quantity the effect of Xe-ELIP on hemorrhage volume and also to evaluate its neuroprotective effects using a SAH brain injury model.
Methods: Xe-ELIP were prepared by a pressurization-freeze method. SAH was induced in Sprague-Dawley rats by endovascular perforation. Rats (n=18) were randomly divided into SAH, SAH with ELIP treatment and SAH with Xe-ELIP treatment groups. Treatments were administrated intravenously at 30 minutes after SAH. Ultrasound was applied over the common carotid artery to trigger Xe release from circulating Xe-ELIP. Hemorrhage volume was quantified by spectrophotometric hemoglobin assay at two hours after SAH. Expression of caspase-3 and positive TUNEL was examined as markers for apoptosis.
Results: SAH without treatment had the greatest hemorrhage volume (327 ± 25μl); the hemorrhage volume in SAH with ELIP treatment group was 274 ± 37μl (p=0.27 vs. no treatment); Xe-ELIP treatment decreased hemorrhage volume to 165 ± 9μl (p<0.001 vs. no treatment; p=0.026 vs. ELIP treatment). Xe-Elip treatment was also associated with reduced inflammation, suppressed TUNEL positive and caspase-3 staining in the cerebral cortex, and improved neurological testing.
Conclusions: Early hematoma growth in subarachnoid hemorrhagic stroke correlates with early brain injury. Xe-loaded liposomes minimizes hemorrhage volume and improves neurological outcomes in SAH. The underline mechanism of Xe hemostasis may relate to its cytoprotectant and anti-inflammatory effects.
Author Disclosures: T. Peng: None. N. Zeng: None. Y. Miao: None. M. Moody: None. D. McPherson: None. S. Huang: None.
This research has received full or partial funding support from the American Heart Association, SouthWest - Arkansas, Colorado, New Mexico, Oklahoma, Texas, Wyoming.
- © 2014 by American Heart Association, Inc.