Abstract T P77: Synergistic Effects of Platelet Lysate and Granulocyte-Colony Stimulating Factor on Safe and Rapid Expansion of Human Bone Marrow Stromal Cells for Ischemic Stroke
Purpose: Human bone marrow stromal cells (hBMSCs) are the most promising donor cells of cell therapy for ischemic stroke. For clinical application, it is essential to establish the protocol to expand the hBMSCs in the animal protein-free medium within significant time limit. This study was aimed to evaluate the effects of human-derived platelet lysate (PL) and granulocyte-colony stimulating factor (G-CSF) on their growth kinetics, neural differentiation, and therapeutic ability in ischemic stroke.
Methods: The hBMSC were cultured in three types of culture media, including FCS-containing medium (hBMSC-FCS), PL-containing medium (hBMSC-PL), and PL and G-CSF-containing medium (hBMSC-PL/G-CSF). Cell growth kinetics was compared among three groups. The rats were subjected to permanent middle cerebral artery occlusion. The hBMSCs-PL, hBMSCs-PL/G-CSF or vehicle was stereotactically transplanted into the ipsilateral striatum at 7 days after the insult. Motor function was serially evaluated throughout the experiments. The distribution and fate of engrafted cells were examined, using fluorescence immunohistochemistry.
Results: The culture with PL and G-CSF significantly shortened the doubling time of hBMSCs. Transplantation of the hBMSCs promoted functional recovery compared with vehicle. The therapeutic effects did not differ between hBMSCs-PL and hBMSCs-PL/G-CSF. Engrafted hBMSCs aggressively migrated toward the infarct rim and some of them acquired the phenotypes of neurons and astrocytes.
Conclusion: These findings strongly suggest that the animal protein-free, pharmacologically modulated protocol enables a safe and quick expansion of hBMSCs for patients with ischemic stroke in clinical situation.
Author Disclosures: T. Yamauchi: None. S. Kuroda: None. H. Saito: None. H. Shichinohe: None. K. Houkin: None.
- © 2014 by American Heart Association, Inc.