Abstract W MP117: Quantitative Susceptibility Mapping Reveals Iron Distribution in a Murine CCM Model
Cerebral cavernous malformations (CCM) are associated with vascular leak and hemorrhage, characterized by the iron-rich hemoglobin breakdown products. Quantitative susceptibility mapping (QSM) is a new noninvasive MRI technique that has demonstrated high sensitivity to evaluate CCM lesion burden. However, accuracy of QSM to depict the iron distribution within individual CCM lesions remains unknown. In this study, we compared lesional iron distribution measured by QSM with histological iron staining in a murine CCM3 model.
Methods: Two Ccm3+/– Trp53–/– mouse brains were scanned on a 3T scanner using a dedicated mouse coil. Serial 1-mm-thick coronal slices of each brain were microsectioned at 5 μm and performed by Perls’ staining for iron deposition. QSM images were reformatted to obtain the same coronal slices prior to comparison. Regions of interest (ROIs) identified iron“patches” on histology (Perls’ blue staining) and QSM images (with a threshold of 1.0 ppm). Cross comparisons were performed to determine the sensitivity and specificity of QSM.
Results: Local susceptibility distribution by QSM correlated well with the iron deposition in tissue based on qualitative comparison, as shown in the Figure below. QSM achieved a sensitivity of 0.8 (p < 0.01) and specificity of 0.94 (p < 0.01).
Conclusions: QSM accurately detects the distribution of iron depositions within CCMs.
Author Disclosures: H. Tan: None. C. Shi: Research Grant; Modest; NINDS. T. Liu: Employment; Modest; MedImageMetric LLC. Research Grant; Modest; NIH. Ownership Interest; Modest; Dr. Liu has filed patent related to QSM technology. R. Edelman: None. I. Awad: Research Grant; Modest; NIH.
- © 2014 by American Heart Association, Inc.