Abstract W P95: Assessment of the Optimal Cell Number for the Trans-arterial Transplantation after Stroke in Rat
Introduction and Purpose: Recently, cell transplantation have emerged as a promising treatment option for cerebral infarction in various kinds of animal models. However, optimal conditions (cell number, timing of cell transplantation, optimal route of delivery, best source of cells) remain unknown. The aim of this study was to clarify the optimal cell number for trans-arterial transplantation after stroke.
Methods: Male Sprague-Dawley rats were subjected to an intraluminal occlusion of the middle cerebral artery (75 min.). At 24 hours post-stroke, we transplanted human mesenchymal stem cells (hMSCs) via carotid artery. The animals were divided into three groups, 1х106 hMSCs transplantation (n=8), 1х106 hMSCs transplantation (n=8) , and vehicle (n=8). Neurological recovery was assessed using modified neurological severity score (mNSS) and cylinder tests at day 0, 1, 2, 5 and 9 post-stroke in addition to body weight, lesion size, immune response, and hMSC distribution.
Results: Rats treated with 1х106 hMSCs improved neurological recovery at day 5 (P<0.05), and day 9 post-stroke (P<0.05). Moreover, rats treated with 1х106 hMSCs cells showed further improvement at day 5 (P<0.01), and day 9 (P<0.05) compared with vehicle group. Transplantation of hMSCs (1х104,1х106) significantly showed less body weight loss at day 8 and 9 post-stroke (P<0.01), and revealed smaller lesion size at day 9 post-stroke (P<0.05). Interestingly, hMSC treated (1х104, 1х106) animals showed better engraftment of cells in the peri-infarct area, where immune response was significantly supressed (P<0.05).
Conclusion: We found that trans-arterial transplantation with small amount of hMSCs could be sufficient to show functional recovery after stroke. These findings provide new information about optimizing cell number in trans-arterial transplantation after stroke, which is much beneficial especially for autologous transplantation.
Author Disclosures: Y. Fukuda: None. N. Horie: None. K. Satoh: None. S. Ishizaka: None. T. Hiu: None. N. Nishida: None. I. Nagata: None.
- © 2014 by American Heart Association, Inc.