Abstract T P230: Multiple Applications of Dodecafluropentane emulsion (DDFPe) Prevents Death in a Rabbit Stroke Model of Permanent Ischemia
Background: Stroke remains the 4th leading cause of death in the US with stroke related deaths occurring every 4 minutes. Perfluorocarbons (PFC) have been used to circulate oxygen and rescue ischemic tissue. Intravenous Dodecafluoropentane emulsion (DDFPe), a PFC, has shown great promise as a neuroprotective agent in embolic ischemic stroke models. Our recent experience in rabbit stroke models normally compares stroke size and neurological effect outcomes, but here we review the overall death rates in the permanent ischemic model in control and DDFPe treated groups. These deaths are sometimes associated with occlusions of posterior branches of the Circle of Willis or angiographic difficulties in the embolization procedure.
Methods: Previous data collected on 195 New Zealand White male or female rabbits (3.8 to 6.6 kg bw) with permanent occlusion of the cerebral arteries with two to three 700-900 micron diameter spheres was further reviewed to determine incidence of mortality in all controls and DDFPe treatment groups. In multiple applications, the DDFPe was administered at 90 min intervals. We studied total number of applications regardless of spasm or specific arterial occlusions, including occlusions in the posterior circulation.
Results: See Table.
Conclusion: Increasing the number of DDFPe applications was significant in reducing the incidence of premature death in a model of permanent ischemic stroke and extending survival to scheduled sacrifice. This new PFC has previously demonstrated decreased stroke volume in the rabbit stroke model and now further indicates its ability to significantly reduce the incidence of death. This warrants further studies with DDFPe use in situations of critical major vessel occlusions, vascular spasm and abnormal vasculature in animal stroke models.
Author Disclosures: A. Brown: None. M.C. Arthur: None. R.D. Skinner: None. J.D. Lowery: None. H.P. Hendrickson: None. W.C. Culp: None.
- © 2015 by American Heart Association, Inc.