Abstract T P61: Network Profile Between Neuroprotector and Immune Responses Following Acute Stroke
INTRODUCTION: The nation’s leading cause of death and disabilities is stroke. The only FDA-approved treatment for ischemic stroke remains tissues plasminogen activator (tPA) within 3-4.5 hours after onset. Humans demonstrate early neuroprotection and immune response prevention in stroke. Neuroglobin (NGB), a promising neuroproteective agent, directly binds with toxic substances and increased the oxygen level within brain tissues after stroke.
PURPOSE: To analyze the network mechanism between neuroprotective and immune responses in critical hours and to identify personalized intervention for individuals with more aggressive conditions.
METHODS: This pilot study included 92 Caucasian stroke subjects (ischemia and hemorrhage), with the following inclusion criteria: 1) adults (age 18-75) diagnosed within 24-72 hours of initial symptoms of stroke; 2) CT/MRI image with clinical biomarker-cerebral ischemia/hemorrhage; 3) NIHSS and mRS data available; and 4) tPA infusion and intra-arterial therapy allowed at Memorial Hermann Hospital, Medical Center in Houston, and IRBs approved. Peripheral blood and DNA extracted samples were obtained from baseline to seven days. Neurological outcomes, NGB ELISA, Western blot, mass spectrometry (MS), immunoprecipitation, and coding sequence were collected.
RESULTS: The average age of stroke was 58.9+12, with NIHSS score 0-40, and mean of admitted SBP/DBP =160/87 mmHg. Earliest Sign/Symptoms included visual (n=60), facial paresis (n=49), motor (n=59) deficiency. Forty-five subjects received tPA within 3-4.5 hours (ICH=41) with 58 had a stroke history; GCS, GOS, NIHSS, mRS, and Barthel were collected. NGB protein expression was higher in ischemic stroke than hemorrhagic stroke. Males had higher NGB protein than females. NGB single nucleotide polymorphisms (rs3783988 and rs10133981) were significantly identified, and MS detected nine peptides associated with NGB. A self-defense pathway was provided.
CONCLUSION: Natural protective phenomena for individuals are critical. Bridging the bench evidence and clinical practice may promote a more enhanced personalized intervention and better identify main predictors. Future direction will focus on acute network mechanism of self-defense prevention.
Author Disclosures: P. Chuang: None.
- © 2015 by American Heart Association, Inc.