Abstract T P88: Impaired Collateral Status During Unilateral Carotid Occlusion In Type 2 Diabetic Mice
Introduction: Type 2 diabetes mellitus (T2DM) is major risk factor of ischemic stroke and is associated with poor outcome after stroke. Previously we found that T2DM mice exhibited impaired leptomeningeal collateral flow recruitment after distal middle cerebral artery occlusion (MCAO). The current study aimed to study the collateral status during unilateral carotid occlusion, commonly observed in human patients with atherosclerosis.
Methods: Adult male db/db and db/+ mice were subjected to permanent unilateral common carotid artery occlusion (CCAO) to simulate the hypoperfusion state in the human carotid artery disease. In vivo imaging of blood flow and flow velocity of the leptomeningeal collateral vessels was performed at various time points after CCAO with doppler optical coherence tomography (DOCT). Diameter of each major artery in the circle of Willis was also quantified by DiI-labeling at various time points after CCAO.
Results: Imaging of DOCT showed that during occlusion of CCA, db/+ mice showed more robust retrograde filling of several distal branches of the MCA from ACA immediately and 24 hours after CCAO compared to db/db mice. Data with DiI-labeling showed that in both strains, the diameters of Willis ring including ICA, MCA, ACA gradually enlarged after 24 hours following CCAO. However the diameters of these vessels of db/db mice on the ischemic side were significantly smaller at 24 hours and 7 days after CCAO compared to that of db/+ mice.
Conclusion: Our results demonstrate an impairment of leptomeningeal collateral recruitment and a deficiency in collateral vessel growth in the circle of Willis after CCAO in db/db mice. These findings might underlie the increased risk of ischemic injury in T2DM patients with carotid disease or stroke.
Author Disclosures: Y. Nishijima: None. Y. Akamtsu: None. C. Lee: None. J. Liu: None.
- © 2015 by American Heart Association, Inc.