Abstract T P95: Stroke Physical Rehabilitation: Impact on Motor Functional Recovery in Drug Treated versus Vehicle Treated Rats
Background: Post-stroke physical rehabilitation has been considered essential for enhancing sensorimotor functional recovery. We assessed the hypothesis that physical rehabilitation would improve functional task performance in drug-treated rats compared to vehicle-treated ones.
Methods: Adult female Sprague-Dawley rats, which have previously been trained on Montoya Staircase and Forelimb Asymmetry tasks, were subjected to endothelin-1-induced middle cerebral artery occlusion. The rats were treated daily using either a drug combination of 5 mg/kg Fluoxetine and 1 mg/kg Simvastatin or a vehicle control beginning 6-12 hours after stroke. On post-stroke day 8, the rats were underwent voluntary rehabilitation every other day for a period of five and half weeks. Behavioral tests were performed to assess motor functional recovery after stroke induction.
Results: In drug-treated rats, both rehabilitated and non-rehabilitated groups showed a 34% recovery in contralateral limb function; whereas, in vehicle-treated rats, animals that underwent physical rehabilitation showed a mean of 26% recovery in contralateral limb function versus 8.6% recovery in non-rehabilitated ones. The ipsilateral limb of non-rehabilitated (control and drug-treated) rats showed a significant (~23% versus -0.1%) difference in functional recovery from rehabilitated rats. There was also significance difference in the baseline ipsilateral deficit between rehabilitation and non-rehabilitation groups which may help explain this statistical difference in the ipsilateral limb performance. The starting time for drug delivery may have negatively influenced the functional recovery. The early delivery (6-12 hours after stroke induction) of this drug combination resulted in a larger infarct size than had previously been seen when this drug treatment was delivered beginning 20-26 hours after stroke induction. Early delivery of the drug combination may result in secondary hemorrhagic stroke.
Conclusion: This study proves that our drug combination treatment allows for sensorimotor functional recovery after ischemic stroke in those individuals for whom physical rehabilitation may not be possible, giving slightly better motor functional recovery in grasping.
Author Disclosures: M.A. Ragas: None. M. Balch: None. A. Hensley: None. K. Reynolds: None. D. Wright: None. B. Kerr: None. A.M. Corbett: None.
- © 2015 by American Heart Association, Inc.