Abstract W P187: Pathological Differences of Ischemic Stroke Caused by Cholesterol versus Clot Embolization
Objectives: The nature and shapes of cholesterol and blood emboli are significantly different. The objective of this study was to investigate if any significant pathological differences between the embolic ischemic strokes resulted from cholesterol versus blood clot embolization. Methods: Embolic stroke model was created using Sprague-Dawley rats. They were randomly divided into three groups: control, cholesterol emboli, and blood clot emboli groups. After written consents were obtained, we collected cholesterol plaque specimens and blood samples from the patients who underwent carotid endarterectomy. Plaques were rinsed and heated at 100oC for 60 minutes to denature protein in the plaques and minimize immunoreaction in animal models. Plaques and blood clots were broken into 0.30 - 0.35 mm and diluted into a final concentration of 1,000 fragments per 100 μL. Normal saline, the suspensions of cholesterol emboli and blood clot emboli (300μL) were separately injected into rat brains through the left internal carotid artery, based on different groups. Rats were sacrificed at 3, 6 and 24 hours post injection and brain ischemia was assessed using pathological H/E staining. Results: Shrunken eosinophilic changes, which are the characteristics of moderate hypoxia/ischemia injury, were identified in hippocampus in both cholesterol and blood clot embolic groups. However, the severe neuron damage with cell necrosis was only visualized in the cholesterol embolic group at 24 hours (Figure 1) Conclusions: The findings suggest that cholesterol and blood clot embolization may result in different degrees of damage in brain tissue. It may affect the treatment choice and the outcome of embolic strokes.
Author Disclosures: S. Yang: None. H. Dong: None. L. Yao: None. X. Gu: None. C. Zhao: None. W. Zhang: None.
- © 2015 by American Heart Association, Inc.