Abstract W P254: Docosahexaenoic Acid Therapy of Experimental Hemorrhagic Stroke
INTRODUCTION: Recently we have shown that novel therapy with docosahexaenoic acid (DHA) and DHA complexed to albumin (DHA-Alb) is neuroprotective after ischemic stroke in rats. The purpose of this study was to determine whether treatment with DHA and DHA-Alb would protect against intracerebral hemorrhagic stroke (ICH).
METHODS: The ICH model was produced in male Sprague-Dawley rats by a double-injection method in which 45 μl of fresh arterial blood was injected into the right striatum. The neurological status was evaluated after ICH (at 50 min) and at 1h, 1, 2, 3 and 7 days after treatment; a grading scale of 0-12 was employed (normal score=0, maximal deficit=12). Six groups we studied: DHA (5 mg/kg), Alb (0.63 or 1.25 g/kg), DHA-Alb (5 mg/kg+0.63 g/kg or 5 mg/kg+1.25 g/kg) or saline were administered i.v. at 60 min after ICH (n=10-12 per group). Ex vivo imaging of the brains was conducted on 11.7T MRI on day 7. High-resolution T2 weighted images (T2WI) and apparent diffusion coefficient maps were generated. After completion of the MRI study, histopathology and immunochemistry were conducted and hematoma volumes were determined.
RESULTS: The physiological variables were entirely comparable among the six groups. Treatment with DHA significantly improved behavior on days 1, 2, 3 and 7 (by 25-32) and reduced hematoma volume by a mean of 33% compared to saline group. Animals treated with low and moderate doses of DHA-Alb showed an improved neurological score compared to corresponding Alb groups on day 1 (23 and 26%), day 2 (32 and 31%), day 3 (34 and 33%) and day 7 (31 and 36%, respectively). MRI revealed a consistent hematoma involving the striatum and overlying corpus callosum. Moderate doses of DHA-Alb reduced hematoma volumes compared to Alb group (by 42%). Low dose of DHA-Alb also reduced hematoma volumes, but it was not statistically different from Alb group. Lesion volumes computed from T2WI images and by histopathology agreed closely with one another and were highly correlated.
CONCLUSION: We concluded that both, DHA and DHA-Alb therapy provides neurobehavioral recovery and reduces hematoma volume when administered at 1h after ICH in rats.
- Cerebrovascular disorders
- Magnetic resonance imaging
- Dose-response relation, drug
- Fish oils
Author Disclosures: L. Belayev: None. L. Khoutorova: None. S. Hong: None. S. Hong: None. A. Obenaus: None.
- © 2015 by American Heart Association, Inc.