Abstract TP403: Sex-Dependent Differences in a Mouse Model of Acta2-Related Cerebrovascular Disease
Moyamoya Disease is two to four times more common in females than males. The underlying mechanisms behind this are currently unknown. Missense mutations in Acta2 predispose affected individuals to a variety of vascular diseases, including Moyamoya-like cerebrovascular disease. In this study we examined vessel wall thickness on H&E stained brain sections from WT and Acta2-/- male and female mice at 12 weeks of age (n=3 per a group). We found that female Acta2-/- mice had significantly greater percent vessel wall thickness compared to male Acta2-/- mice across all vessel sizes. There was not a significant difference between male and female vessel wall thickness in the wild-type groups. These findings in Acta2-/- mice suggest important sex-dependent differences in the function of α-smooth muscle actin (SMA) in the cerebrovascular system, and likely the vascular system as a whole. This has important implications for the design of studies examining the role of SMA in cerebrovascular disease and the investigation of novel therapies.
Figure. Percent wall thickness (%TH) by sex. Two-tailed two-sample t-Test assuming unequal variances: * = p<0.05, ** = p<0.005, *** = p<0.0005 and # = one-tailed t-test significant but not two-tailed. SL=short vessel diameter.
Author Disclosures: J.M. Wright: None. J. Chen: None. D.M. Milewicz: None.
- © 2017 by American Heart Association, Inc.