New Grading System Based on Magnetic Resonance Imaging in a Mouse Model of Subarachnoid Hemorrhage
Background and Purpose—A grading system for experimental subarachnoid hemorrhage (SAH) that does not require animal euthanasia is currently unavailable. We proposed a new grading system based on MRI and evaluated the feasibility of this method in a mouse model of SAH.
Methods—SAH was induced by endovascular perforation in adult male C57BL/6 mice. Mice underwent MRI 24 hours after SAH and were categorized into the following 5 grades based on T2*-weighted imaging: Grade 0, no visible SAH or intraventricular hemorrhage (IVH); Grade 1, minimal/localized SAH without IVH; Grade 2, minimal/localized SAH with IVH; Grade 3, thick/diffuse SAH without IVH; and Grade 4, thick/diffuse SAH with IVH. Neurological deficits were then assessed and the mice euthanized for conventional SAH grading.
Results—Among a total of 47 mice, 4% were scored as grade 0, 30% as grade 1, 11% as grade 2, 30% as grade 3, and 26% as grade 4. This MRI grading had excellent interobserver reliability (weighted κ value =0.94), and there were strong correlations between the MRI grading and the conventional grading (r=0.85; P<0.001) or between MRI grade and neurological scores (r=−0.46; P<0.01).
Conclusions—The new MRI grading correlated well with conventional grading and enabled in vivo evaluation of SAH severity. This grading system may offer advantages in future studies of experimental SAH.
- Received October 17, 2014.
- Revision received December 2, 2014.
- Accepted December 4, 2014.
- © 2014 American Heart Association, Inc.